The principle of UV-Vis spectroscopy is based on the absorption of UV or visible light by chemical compounds [2,3,6] . When UV-Vis light passes through a sample, some wavelengths are absorbed while others are transmitted. The amount of transmitted light is measured as transmittance (T), and absorbance (A) is calculated using. Spectroscopy is the measurement and interpretation of electromagnetic radiation absorbed or emitted when the molecules or atoms or ions of a sample move from one energy state to another energy state. UV spectroscopy is a type of absorption spectroscopy in which light of the ultra-violet region. Ultraviolet (UV) and visible radiation are a small part of the electromagnetic spectrum, which includes other forms of radiation such as radio, infrared (IR), cosmic, and X rays. It is widely applied in chemistry, biochemistry, and environmental science for compound identification and quantification.
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